Research Paper Volume 16, Issue 6 pp 5108—5122

Figure 6. POL inhibited tumor growth in mice in vivo. POL significantly reduced the weight (A) and volume (B) of mouse tumors. POL increased the levels of ROS (C) and MDA (D) in mouse tumor tissues. (E) POL decreased GSH levels in mouse tumor tissues. (F) TUNEL staining revealed that POL increased the cell death rate in tumor tissues (20×). (G) IHC staining revealed that POL increased the level of ACSL4 in tumor tissues (20×). (H) RT-PCR results revealed that POL increased the mRNA levels of ptgs2 as well as chac1 in mouse tumor tissues. (I) Western blot similarly confirmed that POL increased the protein expression level of ACSL4 in mouse tumor tissues. ^**P < 0.01, ^***p < 0.001 vs. Con.