Research Paper Volume 16, Issue 6 pp 5108—5122

Figure 5. Knockdown of ACSL4 reverses POL-induced ferroptosis in ovarian cancer cells. Western blot assay revealed that POL-induced high expression of ACSL4 was reversed to some extent by si-ACSL4 in SKOV3 (A) and Hey (B) cells. EdU staining showed that POL decreased the proliferation of SKOV3 (C) and Hey (D) cells, and si-ACSL4 could reverse this effect (20×). Silencing ACSL4 also reduced POL-induced lipid peroxidation in SKOV3 (E) and Hey (F) cells (40×). ^*P < 0.05, ^**p < 0.01 vs. Con; ^#P < 0.05, ^##P < 0.01 vs. POL.