Research Paper Volume 16, Issue 6 pp 5108—5122
Portulaca oleracea L. polysaccharide inhibits ovarian cancer via inducing ACSL4-dependent ferroptosis
Figure 3. POL induced ferroptosis in SKOV3 and hey cells. CCK-8 assay showed that ferroptosis inhibitor, Fer-1 and DFO, were able to reverse the POL-induced decrease in cell viability of SKOV3 (A) and Hey (B) cells. RT-PCR analysis showed that POL was able to significantly increase the mRNA levels of ptgs2 and Chac1 in SKOV3 (C, D) and Hey (E, F) cells. *P < 0.05, ***p < 0.001 vs. Con; #P < 0.05, ##P < 0.01, ###P < 0.001 vs. POL.