Research Paper Volume 16, Issue 5 pp 4541—4562

Figure 4. NTZF alleviated OGCs senescence and improved antioxidant capacity. (A) The cell proliferation rate was detected by CCK-8. (B, C) Cell proliferation was detected by EdU cell proliferation staining (×200 magnification). The ratio of EdU positive cells = the number of EdU stained cells/the number of DAPI stained cells ×100%. (D–F) The protein expression of PCNA and γ-H2AX was detected by western blot. (G) The levels of PCNA in the ovaries of mice were detected by IHC (×400 magnification). (H) SA-β-gal staining was used to detect cell senescence (×400 magnification). Blue staining indicated cell senescence. (I) The intracellular ROS level was detected by DCFH-DA probe. Green fluorescence indicated ROS expression. (J) The level of intracellular GPX. (K) The level of intracellular SOD content (^*p < 0.05, ^**p < 0.01, ^***p < 0.001, versus control group, n = 3; ^#p < 0.05, ^##p < 0.01, ^###p < 0.001, versus H₂O₂ group, n = 3).