Research Paper Volume 16, Issue 5 pp 4541—4562
Ningxin-Tongyu-Zishen formula alleviates the senescence of granulosa cells on D-galactose-induced premature ovarian insufficiency mice
Figure 3. NTZF alleviated oxidative stress and abnormal Sirt1/p53 signaling pathway in POI mice. (A) The level of GPX in mouse ovary. (B) The level of SOD in mouse ovary. (C, D) The levels of Sirt1 in the ovaries of mice were detected by IHC (×400 magnification). (E, F) The levels of p53 in the ovaries of mice were detected by IHC (×400 magnification). (G–I) The protein expression levels of Sirt1 and p53 in mouse ovaries were detected by western blot. (*p < 0.05, **p < 0.01, ***p < 0.001, versus control group, n = 3; #p < 0.05, ##p < 0.01, ###p < 0.001, versus model group, n =3).