Research Paper Volume 16, Issue 5 pp 4396—4422
A pair of primary colorectal cancer-derived and corresponding synchronous liver metastasis-derived organoid cell lines
Figure 8. Differences between CWH22-2D/CLM22-2D and traditional adherent 2D cultured cell lines (LoVo and SW480). (A) Analysis of proliferation rates with CCK-8 in CWH22-2D, CLM22-2D, LoVo, and SW480 cells. (B) The differentiation characteristics of subcutaneous neoplasia in nude mice derived from LoVo and SW480 (ATCC), and CWH22 and CLM22 from our culture. Two moderately differentiated (CRC16-Org, CLM15-Org), and two poorly differentiated (CRC1-Org, CLM23-Org) organoid-derived subcutaneous neoplastic tissues were used for comparison. (C) Bright-field images and H&E staining of the structures derived from the cells (LoVo, SW480, CRC1-Org, CLM23-Org, CWH22-2D, CLM22-2D, CRC16-Org, and CLM15-Org) cultured in the same 3D culture system. Scale bars, 100 μm.