Research Paper Volume 16, Issue 5 pp 4396—4422
A pair of primary colorectal cancer-derived and corresponding synchronous liver metastasis-derived organoid cell lines
Figure 1. Overview of the procedure. Tissues from primary colorectal cancer and liver metastases were obtained and then divided into three parts: two parts were placed in liquid nitrogen and 4% paraformaldehyde, separately, for molecular and histopathological examinations, and the other part was immediately placed on ice and transported to the laboratory for tumor cell isolation and organoid culture. Whole-exome sequencing and histopathological analysis were conducted to determine the concordance between the tumor organoids and corresponding tumors. The organoids were subjected to STR, karyotype analysis, in vitro drug sensitivity tests, and in vivo animal model construction.