Figure 4 — Lei’s formula attenuates osteoarthritis mediated by suppression of chondrocyte senescence via the mTOR axis: in vitro and in vivo experiments

Research Paper|Volume 16, Issue 5|pp. 4250–4269

Lei’s formula attenuates osteoarthritis mediated by suppression of chondrocyte senescence via the mTOR axis: in vitro and in vivo experiments

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Figure 4.LSF inhibits the H₂O₂-stimulated oxidative stress injury in chondrocytes and modulates the mTOR signaling pathway. (A, B) Gradient concentrations of LSF-containing serum were pre-treated with chondrocytes before being given the appropriate concentration of H₂O₂ for incubation, and the expression of mTOR was detected by Western blotting experiments. (C, D) qRT-PCR was performed to detect the expression of mTOR and S6 in chondrocytes processed with LSF and H₂O₂. All data represent mean ± SD. Compared to the control group, ****P < 0.0001; compared to the H₂O₂-stimulated group, ***P < 0.001, ****P < 0.0001.