Research Paper Volume 16, Issue 5 pp 4250—4269

Figure 2. Effect of LSF on chondrocyte viability in H₂O₂-stimulated chondrocytes. (A) Effects of different concentrations of LSF- containing serum acting for different times on chondrocyte viability as determined by CCK8. (B, C) Chondrocytes were pre-treated with LSF-containing serum at screened gradient concentrations before being given the appropriate concentration of H₂O₂ for incubation, as assessed by CCK8. (D, E) The treated chondrocytes were double-stained with Annexin V-FITC/PI and then subjected to flow cytometry to assess the effect of gradient concentration of LSF-containing serum on the apoptotic rate of chondrocytes. (F–H) The treated chondrocytes were cultured in microspheres (7 and 14 days) and stained with Alcian blue to assess the differentiation ability of chondrocytes, and the intensity of the blue color represented the differentiation ability of chondrocytes, and the absorbance was detected by an enzyme marker set at a wavelength of 620 nm. All data represent mean ± SD. Compared to the control group, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001; compared to the H₂O₂-stimulated group, ****P < 0.0001; compared to the same concentration of blank serum group, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.