Research Paper Volume 16, Issue 5 pp 4224—4235
The effect of STAT1, miR-99b, and MAP2K1 in alcoholic liver disease (ALD) mouse model and hepatocyte
Figure 4. MAP2K1 is negatively correlated with miR-99b and downregulated in ALD model. (A) The correlation of MAP2K1 and miR-99b was analyzed using bioinformatic analysis. (B) The expression of MAP2K1 was detected by Western blot analysis in ALD mice. (C) The expression of MAP2K1 was detected by qPCR in ALD mice. (D) The expression of MAP2K1 was measured by qPCR in AML-12 cells treated with 100 mM EtOH. (E) The correlation of MAP2K1 with ALT levels was analyzed in ALD mice. *P < 0.05.