Research Paper Volume 16, Issue 5 pp 4224—4235
The effect of STAT1, miR-99b, and MAP2K1 in alcoholic liver disease (ALD) mouse model and hepatocyte
Figure 3. STAT1 enhances miR-99b expression in ALD model. (A) The correlation of STAT1 and miR-99b was analyzed using bioinformatic analysis. (B) The correlation of STAT1 and miR-99b was measured by qPCR in ALD mice. (C, D) The expression of STAT1 was detected by Western blot analysis in ALD mice. (E, F) The AML-12 cells were transfected with STAT1 overexpressing plasmid and the expression of miR-99b (E) and pri-miR-99b (F) was determined in the cells. (G) Prediction of STAT1 binding site on miR-99b promoter region. (H) The enrichment of STAT1 on miR-99b promoter was identified by ChIP analysis in AML-12 cells. *P < 0.05.