Research Paper Volume 16, Issue 4 pp 3989—4013
Unlocking the potential of senescence-related gene signature as a diagnostic and prognostic biomarker in sepsis: insights from meta-analyses, single-cell RNA sequencing, and in vitro experiments
Figure 8. TGFBI and MAD1L1 were associated with monocyte and B cell senescence, respectively. (A, B) Clustering and cell type annotation of cells isolated from healthy control (A) and sepsis (B) subjects. (C, D) Levels of TGFBI and MAD1L in different cells isolated from control (C) and sepsis (D) subjects. (E–H) Levels of TGFBI in monocytes from control and sepsis subjects (E), and levels of MAD1L1 in B cells (F), NK cells (G), and T cells (H) from control and sepsis subjects. (I, J) Positive association between TGFBI with oxidative stress-induced senescence in monocytes (I), and MAD1L with oxidative stress-induced senescence in B cells (J). (K, L) Up-regulation of TGFBI in THP-1 cells treated with H2O2 (K), and up-regulation of MAD1L1 in IM-9 cells treated with H2O2 (L). Abbreviations: scRNA-seq: single-cell RNA sequencing; *P < 0.05; **P < 0.01; NS: not significant.