Research Paper Volume 16, Issue 5 pp 4191—4203
Calycosin (CA) inhibits proliferation, migration and invasion by suppression of CXCL10 signaling pathway in glioma
Figure 4. Downregulating CXCL10 enhances the effects of calycosin on cell proliferation, migration, invasion and CXCL10 signaling in GBM. (A–D) Effects of CXCL10 knockdown on calycosin in U87 and U251 cell proliferation. (E–H) Effects of downregulating CXCL10 on calycosin in U87 and U251 cell migration and invasion. (I) Downregulating CXCL10 promotes calycosin-induced CXCL10, NLRP3, NF-κB and IL-1β downregulation. Control: siRNA negative control transfection; CA: siRNA negative control transfection + 200μM calycosin; CXCL10: CXCL10 siRNA transfection; both: CXCL10 siRNA transfection + 200μM calycosin. *P < 0.05 vs control. #P < 0.05, compared with either CA treatment or CXCL10 siRNA alone.