Research Paper Volume 16, Issue 4 pp 3803—3822

Figure 9. Expression of PDK2, CHDH, and ALDH5A1 in LPS-induced HIEC-6 cells and DSS-induced colitis mice. The relative mRNA (A) and protein (B) expression levels of PDK2, CHDH, and ALDH5A1 were conducted using the LPS-induced HIEC-6 cells. (C) Photographs were utilized to assess the variations in colon lengths among the groups. (D) The length of the colon in different groups. (E, F) Hematoxylin and eosin staining images and colonic histological scores of colon cross-sections in mice (n = 5). Scale bar = 50 μm. (G) Expression of PDK2, CHDH, and ALDH5A1 in the colon tissues of mice was detected using Western blotting. (H) Double immunofluorescence staining of PDK2 (labeled in green), CHDH (labeled in green), and ALDH5A1 (labeled in green) with M1 macrophages (CD86, labeled in red) at DSS induced mice. The nuclei were stained by DAPI (blue). n = 5 per group. Scale bar = 50 μm. Values are expressed as the mean ± sd (n = 5). ^*P < 0.05, ^**P < 0.01, ^***P < 0.001. Abbreviation: DSS: dextran sodium sulfate.