Research Paper Volume 16, Issue 4 pp 3716—3733

Figure 4. MiR-557 targets RAB10 to affect the biological behaviour of cells. (A–D) Relative expression of RAB10 in mimic-NC cells and miR-557-overexpression cells of MHCC-97H, and in inhibitor-NC cells and miR-557-inhibition cells of Huh7 cells. (E) The relationship between the expression level of miR-557 and the expression level of RAB10 in patients. (F) The relative dual-luciferase reporter assays in four different groups. (G) CCK-8 assays of NC cells, miR-557-overexpression cells, RAB10-overexpression cells, co-transfected miR-557 mimics and pmirGLO-RAB10 cells of MHCC-97H. (H) CCK-8 assays of NC cells, miR-557-down-expression cells, RAB10-down-expression cells, co-transfected miR-557 inhibitors and siRAB10 cells of Huh7. (I, J) Transwell assays of NC cells, miR-557-overexpression cells, RAB10-overexpression cells, co-transfected miR-557 mimics and pmirGLO-RAB10 cells of MHCC-97H, Scale bar: 100 μm. (K, L) Transwell assays of NC cells, miR-557-down-expression cells, RAB10-down-expression cells, co-transfected miR-557 inhibitors and siRAB10 cells of Huh7, Scale bar: 100 μm. (M, N) The distribution phases of cell cycle of NC cells, miR-557-overexpression cells, RAB10-overexpression cells, co-transfected miR-557 mimics and pmirGLO-RAB10 cells of MHCC-97H. (O, P) The distribution phases of cell cycle of NC cells, miR-557-down-expression cells, RAB10-down-expression cells, co-transfected miR-557 inhibitors and siRAB10 cells of Huh7. All blots were cut prior to hybridisation with antibodies during western blotting. (^*P<0.05, ^**P<0.01, ^***P<0.001, ^****P<0.0001).