Research Paper Volume 16, Issue 4 pp 3716—3733

Figure 2. Effect of miR-557 on HCC cell proliferation, migration, invasion, cell cycle, and EMT. (A) Relative expression of miR-557 in different HCC cells lines. (B–E) Relative miR-557 expression in HCC cells that overexpressed miR-557, in MHCC-97H cells, and knockdown miR-557 in Huh7 cells. (F, G) CCK8 assays of mimic-NC cells and miR-557-overexpression cells of MHCC-97H, and CCK8 assays of inhibitor-NC cells and miR-557-inhibition cells of Huh7 cells. (H, I) Transwell assays of mimic-NC cells and miR-557-overexpression cells of MHCC-97H, and transwell assays of inhibitor-NC cells and miR-557-inhibition cells of Huh7 cells, Scale bar: 100 μm. (J–M) The protein and mRNA of EMT process of mimic-NC cells and miR-557-overexpression cells of MHCC-97H, and of inhibitor-NC cells and miR-557-inhibition cells of Huh7 cells. (N, O) Clone formation assay of Lv-miR-NC cells and Lv-miR-557-overexpression cells of MHCC-97H, and Clone formation assay of Lv-sponge-NC cells and Lv-sponge-miR-557-inhibition cells of Huh7 cells. (P) Orthotopic transplantation tumor formation with Lv-miR-NC and Lv-miR-557 overexpression in MHCC-97H cells or Lv-sponge-NC and Lv-sponge-miR-557-inhibition cells of Huh7 cells. (Q, R) The distribution phases of cell cycle of mimic-NC cells and miR-557-overexpression cells of MHCC-97H, and of inhibitor-NC cells and miR-557-inhibition cells of Huh7 cells. All blots were cut prior to hybridisation with antibodies during western blotting. (S, T) The tumor weight analysis of mimic-NC cells and miR-557-overexpression cells of MHCC-97H, and of inhibitor-NC cells and miR-557-inhibition cells of Huh7 cells. (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001).