Research Paper Volume 16, Issue 4 pp 3612—3630
New insights into ginsenoside Rg1 regulating the niche to inhibit age-induced germline stem cells depletion through targeting ECR/BMP signaling pathway in Drosophila
Figure 5. Effects of ginsenoside Rg1 on GSCs niche in ECR mutant Drosophila. (A) GSCs are labeled with α-spectrin antibody (red, fusions), Vasa antibody (green, germ cells), and DAPI (blue, nuclei), Scale bar: 10 μm; (B) Average number of GSCs; (C) Relative mRNA-level expression of gene E-cadherin (n = 100); (D) Western blotting analysis of expression of Bam and Nos; (E) Relative expression levels of Bam (n = 100); (F) Relative expression levels of Nos (n = 100); GAPDH antibody was used as loading Control. Results were analysed with one-way ANOVA. Data are shown as the mean ± SD (n = 100); ###p < 0.001 compared with Control (7-day-old female Drosophila); ***p < 0.001 compared with Model (35-day-old female Drosophila); +++p < 0.001 compared with Control (7-day-old female Drosophila); Abbreviation: ns: no significance.