Figure 3. Effect of ginsenoside Rg1 on niche BMP signaling pathway. (A) Western blotting analysis of expression of ISWI, Dpp and Smad 4; (B) Relative expression levels of ISWI; (C) Relative expression levels of Dpp; (D) Relative expression levels of Smad4; (E) Western blotting analysis of expression of pSmad1/5/8; (F) Relative expression levels of pSmad1/5/8; GAPDH antibody was used as loading Control. Results were analysed with one-way ANOVA. Data are shown as the mean ± SD (n = 100); ###p < 0.001 compared with Control (7-day-old female Drosophila); *p < 0.05, **p < 0.01, ***p < 0.001 compared with Model (35-day-old female Drosophila).