Research Paper Volume 16, Issue 4 pp 3583—3595
Rutin promotes osteogenic differentiation of mesenchymal stem cells (MSCs) by increasing ECM deposition and inhibiting p53 expression
Figure 7. Rutin inhibits TP53 (p53) activity and promotes ECM deposition during osteogenic induction. (A, B) The protein expression of fibronectin, COL-I and integrin β1 in MSCs of the rutin and control groups. (C) Real-time RT-PCR analysis showed that the expression of ECM-related makers (fibronectin, COL-I) and integrin β1 were significantly enhanced by rutin treatment. (D, E) The protein expression of p65, p53, and cleaved caspase3 in MSCs of the rutin and control groups. The quantitative data were expressed as the mean ± S.D. of three independent experiments (*p < 0.05).