Research Paper Volume 16, Issue 4 pp 3554—3582

Figure 9. SLC30A7 suppressed cuproptosis via activating the JAK2/STAT3/ATP7A pathway. (A) Cu²⁺ levels in cytoplasm, mitochondria and medium were measured using Copper (Cu) Colorimetric Assay Kit (Elabscience, E-BC-K300-M). (B) Western blot showing the protein level of ATP7A following SLC30A7 knockdown in GBM cells. (C) GSEA analysis showed that the high expression SLC30A7 group was positively correlated with the JAK-STAT pathway. (D, E) Western blot analysis showed that SLC30A7 activated the JAK2/STAT3 pathway. (F) Western blot analysis demonstrated that JAK2/STAT3 pathway promoted APT7A protein levels in GBM cells. Conversely, inhibitor of JAK2/STAT3 signaling partially rescued APT7A protein levels in GBM cells. (G) Colony formation assay of the effect of WP1066 on the growth of GBM cells after overexpression of SLC30A7. (H) Transwell migration analysis showing the effect of WP1066 on GBM cells after overexpression of SLC30A7 on GBM cells.