Figure 9.ACTC1 knockdown in vitro experiment. (A) RT-qPCR analysis was performed to verify the knockdown of ACTC1. (B) Expression analysis of ACTC1 in 20 pairs of BRCA tissue samples. (C, D) CCK-8 experiments. The activity of MDA-MB-231 and MCF-7 cell lines decreased significantly. (E) The cloning ability of MDA-MB-231 and MCF-7 cell lines decreased significantly. (F) EdU test. After ACTC1 knockdown, the proliferation ability of MDA-MB-231 and MCF-7 cell lines decreased significantly. (G) Healing test. After ACTC1 knockdown, the migration ability of MDA-MB-231 and MCF-7 cell lines decreased significantly. (H) Transwell assay. The migration and invasion abilities of MDA-MB-231 and MCF-7 cell lines were significantly decreased (*P<0.05, **P < 0.01, P***<0.001).
