Research Paper Volume 16, Issue 4 pp 3363—3385
Pan-cancer analysis of NUDT21 and its effect on the proliferation of human head and neck squamous cell carcinoma
Figure 8. Knockdown of NUDT21 increases apoptosis in FaDu and CNE-2Z cells and blocks the cell cycle of FaDu cells. (A, B) Cell death was determined by Annexin-V staining and flow cytometry, and shNUDT21-treated cells showed a significant increase in apoptosis compared to shCtrl-treated cells. (C, D) Measurement of Caspase 3/7 activity. shNUDT21-treated cells showed a significant increase compared to shCtrl-treated cells. (E) Cell cycle analysis of FaDu cells by flow cytometry. shNUDT21-treated cells showed a significant decrease in G1 and a significant increase in G2/ M compared to shCtrl-treated cells. (shCtrl: control lentivirus; shNUDT21: lentivirus containing shRNA targeting NUDT21). **p<0.01 compared to shCtrl. (F, G) Modification of IkBa (Total), p-IkBa (Ser32/36), Survivin (Total), cleaved PARP, cleaved Caspase-3, p-Bad in shNUDT21-treated cells. (H) Network of NUDT21-related molecular proteins in cells. **p<0.01 compared to shCtrl.