Research Paper Volume 15, Issue 24 pp 15599—15623
Unravelling diagnostic clusters and immune landscapes of cuproptosis patterns in intervertebral disc degeneration through dry and wet experiments
Figure 12. Immunofluorescence analysis and RT-qRCR validation in vitro. (A) Representative immunofluorescence images of ACAN in NP cells photographed by fluorescence microscopy; Scale bar = 100 μm. (B–K) Cuproptosis genes FDX1, LIAS, LIPT1, GCSH, DLST, DLAT, and PDHB exhibited significantly lower expression levels in IDD NP cell samples than controls, while ATP7A, ATP7B and MTF1 displayed the opposite results. All results were expressed as mean ± standard deviation. *p < 0.05; **p < 0.01; ***p < 0.001. Abbreviations: CTR: control; TBHP: tert-butyl hydrogen peroxide.