Research Paper Volume 16, Issue 1 pp 844—856

Figure 5. Reversal of autophagy insufficiency in POI-GCs by melatonin via forkhead box O-3A (FOXO3A) signaling. (A) Gene expression of FOXO3A in GCs from POI and normal patients (n = 4-6). (B) Representative WB results of FOXO3A in GCs from POI and normal patients (n = 3). (C) Representative WB of FOXO3A in POI-GCs after MT treatment (n = 3). (D) Representative IF staining and analysis of autophagy in POI-GCs after FOXO3A knockdown with or without MT (n = 5). (E) Representative EDU staining results and analysis for cell proliferation in POI-GCs after MT and siFOXO3A treatment (n = 5). Scale bar, 100 μm. (F) Flow cytometry analysis of apoptotic cell rates in POI-GCs after MT and siFOXO3A treatment (n = 4). (G) Analysis of ROS production in POI-GCs after MT and siFOXO3A treatment (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001.