Figure 3.Regulation of autophagy in POI-GCs by melatonin (MT). (A) ELISA analysis of MT levels in GCs (n = 4-6). (B) Representative WB and analysis of autophagy-related proteins in POI-GCs after MT treatment (n = 3). (C) Representative IF staining and analysis of autophagy flux in POI-GCs after MT treatment (n = 5). Scale bar, 50 μm. *p < 0.05, **p < 0.01, ***p < 0.001.
