Research Paper Volume 16, Issue 2 pp 1298—1317

Figure 7. EGR1 directly binds to the miR-96-5p promoter region. (A) Sequence logo representing the consensus EGR1 binding motif from JASPAR database (http://jaspar.genereg.net/). (B) miR-96-5p promoter region was analyzed from the TSS. The mutation was seen from -1363~-1350. (C) The luciferase activity was detected in NSCLC cells after transfection with the EGR1-expressing vector. The luciferase reporter plasmid contained a 2 kb fragment upstream of the miR-96-5p stem-loop. (D) Theoretical binding mode of α-Hederin at the binding site of EGR1. (E) Western blot demonstrating EGR1 protein levels in nuclear and cytosol in NSCLC cells after treatment with α-Hederin for 24 hours. (n = 3). Data are shown as mean ± SD, One-way ANOVA.