Research Paper Volume 16, Issue 2 pp 1298—1317
α-Hederin promotes ferroptosis and reverses cisplatin chemoresistance in non-small cell lung cancer
Figure 6. miR-96-5p directly binds to DDIT3 in NSCLC cells. (A) The predicted binding sites of miR-96-5p in DDIT3. The mutated (Mut) version of DDIT3 is also presented. (B) Relative luciferase activity in A549/DPP and PC-9/DPP cells was measured 48 h after transfection. (C) qRT-PCR demonstrating miR-96-5p levels in NSCLC cells after treatment with α-Hederin for 24 hours. (D, E) qRT-PCR demonstrating miR-96-5p, DDIT3, and ATF3 levels in NSCLC cells after transfection with miR-96-5p mimic or miR-96-5p inhibitor for 24 hours. (F) Western blot demonstrating DDIT3 and ATF3 levels in NSCLC cells after transfection with miR-96-5p mimic or miR-96-5p inhibitor for 24 hours. (n = 3). Data are shown as mean ± SD, One-way ANOVA.