Research Paper Volume 16, Issue 2 pp 1298—1317

Figure 4. α-Hederin treatment activated ferroptosis in NSCLC. (A) OPLS-DA score plot showing differences between α-Hederin treatment and control groups in A549/DPP cells. (B) A heat map was used to identify metabolites in A549/DPP cells. (C) Cellular GSH level was detected in NSCLC cells after treatment with α-Hederin for 24 hours. (D) Cellular NADPH level was detected in NSCLC cells. (E) Cellular ROS level was detected in NSCLC cells. (F) Immunofluorescence assays were performed using C11-BODIPY (green) to assess oxidation and DAPI staining to visualize the Nuclei (blue) in NSCLC cells. (G) Western blot demonstrating SLC7A11 and GPX4 protein levels in NSCLC cells after treatment with α-Hederin for 24 hours. (H, I) qRT-PCR demonstrating SLC7A11 and GPX4 mRNA levels in NSCLC cells. (n = 3). Data are shown as mean ± SD, One-way ANOVA.