Research Paper Volume 16, Issue 1 pp 617—626

GDF15 restrains myocardial ischemia-reperfusion injury through inhibiting GPX4 mediated ferroptosis

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Figure 5. pc-DNA-GDF15 significantly inhibited the ferroptosis in OGD/R-treated H9C2 cells. (A, B) Measurement of cell migration with wound healing assay (bar: 400 μm, magnification: 10×); (C) Detection of cell proliferation with CCK8; (D, E) Measurement of cell invasion with Transwell assay (bar: 100 μm, magnification: 100×); (F, G) Measurement of cell migration with flow cytometry. *P < 0.05, **P < 0.01, ***P < 0.001.