Research Paper Volume 15, Issue 23 pp 13628—13645

Figure 7. SIRT6 activation limits senescence burden in murine cartilage explants. (A) Femoral cap cartilage was obtained from each hindlimb of 3-week-old p16^tdTom mice and cultured for three weeks under senescence inducing conditions with either 20 μM MDL-800 or DMSO control. Analysis of the percentage of cells positive for tdTomato performed by flow cytometry. Data from Cohort 1 were normally distributed by Shapiro-Wilk and thus paired t-test was applied. (B) Same as panel A but a different cohort of mice. Data from cohort 2 were not normally distributed by Shapiro-Wilk and thus Wilcoxon matched-pairs signed rank test was applied. (C) For each mouse (blue circles: cohort 1; red triangles: cohort 2), the percentage of tdTomato-positive cells in the MDL explant was normalized to the DMSO explant. Asterisks denote statistical significance, with ^*p < 0.05, ^***p = 0.001.