Research Paper Volume 15, Issue 23 pp 14457—14472
MiR-203a-3p attenuates apoptosis and pyroptosis of chondrocytes by regulating the MYD88/NF-κB pathway to alleviate osteoarthritis progression
Figure 1. miR-203a-3p was decreased, and MYD88 was increased in LPS-treated chondrocytes. (A, B) qPCR showing miR-203a-3p expression in primary rat chondrocytes treated with different concentration of LPS (0, 1, 2.5,5 and 10 μg/ml) for 24 hours and treated with 10 μg/ml at different time points (0, 2,4,8,12 and 24 hours). (C) qPCR showing MYD88 mRNA expression in primary rat chondrocytes treated with different concentration of LPS for 24 hours. (D, E) WB analysis and relative quantification showing the protein expression of MYD88 in primary rat chondrocytes treated with different concentration of LPS for 24 hours. All experiments were performed in triplicated and data were presented as the mean±SD, n=3 per group. *P<0.05, **P<0.01.