Research Paper Volume 16, Issue 1 pp 299—321
METTL3-dependent N6-methyladenosine modification is involved in berberine-mediated neuroprotection in ischemic stroke by enhancing the stability of NEAT1 in astrocytes
Figure 6. METTL3 enhances NEAT1 stability in OGD/R astrocytes by modulating m6A modification of NEAT1. (A) Prediction of potential m6A targets in Lnc NEAT1 based on SRAMP online website. (B) MeRIP-PCR to detect the m6A modifications in Lnc NEAT1. (C) Application of MeRIP-PCR to detect the correlation between NEAT1 and METTL3. (D) Western blot to detect expression of METTL3 after overexpression or silencing in primary astrocytes. (E) A bar presenting the quantification of METTL3 in primary astrocytes. (F) Expression of NEAT1 after METTL3 overexpression and silencing in primary astrocytes of mice after OGD/R. (G) Stability of NEAT1 in primary astrocytes with METTL3 overexpression after OGD/R treatment. Data are represented as mean ± SD, (n = 3; *P < 0.05; **P < 0.01; ***P < 0.001).