Research Paper Volume 16, Issue 1 pp 299—321
METTL3-dependent N6-methyladenosine modification is involved in berberine-mediated neuroprotection in ischemic stroke by enhancing the stability of NEAT1 in astrocytes
Figure 5. Berberine exerts neuroprotective effects through modulation of the NEAT1/ miR-377-3p /Nampt axis in astrocytes. (A) RT-qPCR to analyze the NEAT1 level in primary astrocytes transfected with sh-NEAT1 after berberine treatment. (B) RT-qPCR to analyze the miR-377-3p level in primary astrocytes transfected with sh-NEAT1 after berberine treatment. (C) Western blot analysis of Nampt expression in primary astrocytes transfected with sh-NEAT1 after berberine treatment. (D) A bar presenting the quantification of Nampt in primary astrocytes. (E) CCK-8 assay to assess the effect on co-cultured neurons by treating with NEAT1 knockdown and berberine-treated primary astrocytes and OGD/R treatment. (F) LDH assay to study the effect on co-cultured neurons by treating with NEAT1 knockdown and berberine-treated primary astrocytes and OGD/R treatment. The relative expression levels were quantified by normalizing to β-actin. Data are represented as mean ± SD, (n = 3; *P < 0.05; **P < 0.01; ***P < 0.001).