Research Paper Volume 16, Issue 1 pp 299—321
METTL3-dependent N6-methyladenosine modification is involved in berberine-mediated neuroprotection in ischemic stroke by enhancing the stability of NEAT1 in astrocytes
Figure 1. Berberine alleviates MCAO/R brain injury and up-regulates the expression of Nampt in adult mouse astrocytes. (A) Neurologic function scores for assessing the degree of neurological impairment after 24 hours in sham, MCAO/R+Vehicle or BBR+ MCAO/R group of mice (n=24). (B) Representative images of TTC-stained brain sections from mice (n=6). (C) Quantification of infarct volume at 24 h after MCAO/R (n=6). (D) Representative images of HE staining of ischemic brain tissue from mice (n=6, Scale bar: 50 μm). (E) Representative images of Nissl staining of ischemic brain tissue from mice (n=6, Scale bar: 50 μm). (F) Quantification of damaged neurons. (G) Quantification of Nissl+ neurons. (H) Representative Western blot images depicting Nampt in adult mouse astrocytes (n=6). (I) A bar presenting the quantification of Nampt (n=6). The relative expression levels were quantified by normalizing to β-actin. Data are represented as mean ± SD, (*P < 0.05; **P < 0.01; ***P < 0.001).