Research Paper Volume 16, Issue 1 pp 246—266

Figure 3. (A) Upon finalizing the pan-cancer and methylation studies of the Purinergic gene, we embarked on a clustering analysis of all data samples, grounded on the mRNA expression profiles of this gene. The clustering segmented the samples into three distinct groups. Subsequent analyses of these groups encompassed canonical oncogene studies, drug sensitivity evaluations, and immune infiltration assessments. (B) Purinergic scores were calculated based on the level of mRNA expression of Purinergic genes. KIRC samples were divided into three groups according to different levels of Purinergic scores: low expression group (cluster 1), normal expression group (cluster 2), and high expression group (cluster 3). The brown color in the right color bar indicates increased mRNA expression, the gray color indicates no change in mRNA expression, and the blue color indicates decreased mRNA expression. The closer the Purinergic score is to 0.4, the redder the color is, and the closer it is to -0.4, the bluer the color is. The KIRC samples were divided into three groups by cluster analysis; red, green, and black represent cluster 1, cluster 2, and cluster 3, respectively. (C) The violin plot shows the enrichment scores of the three clustered samples, the results show C3 > C2 > C1, and the p-values for comparison between groups are shown in the figure. (D) Survival curve analysis of the three clustered samples. The results show that the survival rate of KIRC patients in the C1 group is much lower than that of KIRC patients in the C2 and C3 groups. Red represents the C1 group, green represents the C2 group, and black represents the C3 group. The horizontal coordinate unit is the number of years of survival, and the vertical coordinate unit is the probability of survival. (E) Heat map showing the association between the three clustered samples and HDAC, SIRT, and classical oncogenes expression, respectively. The color bar red represents high expression, and blue represents low expression. In the legend, red represents cluster 1, green represents cluster 2, and black represents cluster 3.