Research Paper Volume 16, Issue 1 pp 226—245

Figure 7. Detection of CD11b+ cells and RBMX through fluorescence immunohistochemistry assay, and in vitro validation of the functions of RBMX in regulating the proliferative, migrative, and invasive abilities of LIHC cells. (A) Co-fluorescence immunohistochemical analysis of RBMX and CD11b in tumor regions and adjacent normal tissues in three LIHC samples. (B) Western blots showing the RBMX knockdown effects of the three shRNAs in HCCLM3 and SK-HEP1 cells. (C, D) RBMX knockdown reduced the ability of HCCLM3 and SK-HEP1 cell lines for cell colony formation. (E, F) CCK8 assay also validated that RBMX knockdown can reduce the proliferation of HCCLM3 and SK-HEP1 cells. (G–L) Downregulation of RBMX also inhibited the invasive (G, H) and migratory (I–L) abilities of HCCLM3 and SK-HEP1 cells in vitro. CCK8, Cell Counting Kit-8; LIHC, liver hepatocellular carcinoma; RBMX, RNA binding motif protein X-linked; shRNAs, short hairpin RNAs.