Research Paper Volume 16, Issue 1 pp 191—206

Figure 5. Potent antitumor effect of Mn²⁺ and IL-12 combination treatment. (A–C) Mice were subcutaneously inoculated with ID8 cells on day 0. Tumor-bearing mice were randomly divided into four groups: isotype control, Mn²⁺, IL-12, and a combination of Mn²⁺ and IL-12 (n = 5 mice per group). Treatment started on day 6. Body weight changes and tumor growth curves for each mouse are displayed. (D) Tumor tissues were fluorescently stained green, red, and blue to determine cell viability with TUNEL, with Ki-67, and to identify the nuclei, respectively (scale bar, 50 μm).