Research Paper Volume 16, Issue 1 pp 191—206

Figure 1. Mn²⁺ enhanced macrophage STING activity and antitumor capacity in vitro. (A–D) Macrophage STING activity related to treatments with different metal ions; the marker proteins in downstream pathways were immunoblotted. (E) Macrophages were incubated with different metal ions for 24 h, and the response to IFN-β secretion was quantified. (F) qRT-PCR analysis of CD80 and CD86 gene expression. (G, H) To quantify the phagocytic sis of macrophages to tumor cells by flow cytometry, we co-cultured BMDM with pHrodo-labeled tumor cells (ID8) for 24 h, and further observed the effect of macrophages on tumor cells by flow cytometry. One representative experiment is presented from at least three independent experiments, each performed in triplicate. Error bars represent SEM. Data were analyzed by unpaired t-test. Abbreviation: NS: not significant. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001.