Research Paper Volume 16, Issue 1 pp 106—128

Figure 6. The effects of JPYSF on the expression of NAD⁺ biosynthesis-related enzymes in mouse kidney. (A) Pathways of NAD⁺ biosynthesis. (B) Representative Western blot images of QPRT, NAPRT1, NMNAT1, NRK1, and NAMPT in mouse kidney. (C–G) Quantitative analysis of QPRT, NAPRT1, NMNAT1, NRK1, and NAMPT expression normalized to α-Tubulin or GAPDH content (n = 6). (H) Representative immunofluorescence images of QPRT and NAPRT1. (I) Representative immunofluorescence images of NMNAT1 and NRK1. Green corresponds to interest proteins, and blue corresponds to nuclear staining. All images are shown at identical magnification, ×200, scale bar = 100 μm. Data are expressed as mean ± SEM (^nsp > 0.05, ^*p < 0.05, ^**p < 0.01, ^***p < 0.001 between the indicated two groups).