Research Paper Volume 16, Issue 1 pp 106—128

Jian-Pi-Yi-Shen formula alleviates renal fibrosis by restoring NAD+ biosynthesis in vivo and in vitro

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Figure 5. The effects of JPYSF on NAD+ content in vivo and in vitro. (A) NAD+ content in the kidney of normal mice and CKD mice with or without JPYSF treatment (n = 4). (B) NAD+ content in HK-2 cells stimulated with TGF-β1 at the concentrations of 0, 2, 5, and 10 ng/mL (n = 3). (C) NAD+ content in HK-2 cells with TGF-β1 or/and JPYSF stimulation (n = 3). Data are expressed as mean ± SEM (nsp > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001 between the indicated two groups).