Research Paper Volume 15, Issue 22 pp 13558—13578

Figure 2. Upregulation of PTCHD4-AS suppressed GC cell proliferation in vitro. (A) Overexpression efficiencies of PTCHD4-AS was analyzed by qPCR in SGC7901 and MGC803. (B) Viability of SGC7901 and MGC803 cells stably overexpressing EV or PTCHD4-AS. (C) Representative images of EdU in SGC7901 and MGC803 cells stably overexpressing EV or PTCHD4-AS and statistical analysis of Edu-positive cells. (D) Expression of p53 and p21, key proteins of the cell cycle pathway, was detected in the indicated cells by western blotting. (E, F) Cell cycle analysis by FACS in SGC7901 and MGC803 cells stably expressing EV or PTCHD4-AS. (A–F) EV indicates cells transfected with blank vector and PTCHD4-AS indicates cells transfected with lncRNA PTCHD4-AS. Data are presented as mean ± SD, * P < 0.05, ** P < 0.01, *** P < 0.001.