Research Paper Volume 15, Issue 23 pp 14306—14322

Figure 4. miR-299-5p targets DOK7. (A) DOK7-targeting miRNA candidates predicted by “starbase”, “Targetscan” and “miRDB” databases; (B) RT-qPCR analysis of DOK7 mRNA levels after the transfection of miR-NC or the miRNA mimics of hsa-miR-485-5p, hsa-miR-513a-5p, hsa-miR-299-5p, hsa-miR-3194-5p, hsa-miR-378g, hsa-miR-3194-3p and hsa-miR-5691; (C) The predicted binding site of miR-299-5p and DOK7 mRNA 3'UTR region by “TargetScan”; (D) Luciferase reporter assay to detect the binding ability of miR-299-5p to wild-type DOK7 and mutant DOK7 luciferase reporter; (E) WB detection of DOK7 protein levels in different groups (miR-NC, miR-299-5p mimic) of T24 and RT4 cell lines; (F) RT-qPCR detection of miR-299-5p expression in 108 pairs of BLCA cancer tissues and adjacent normal tissues; (G) Spearman correlation coefficient analysis of the relationship between DOK7 and miR-299-5p expression levels in BLCA tumor tissues; (H) RT-qPCR detection of miR-299-5p expression levels in BLCA cell lines and normal bladder cell line. ^***P < 0.001.