Research Paper Volume 15, Issue 23 pp 14292—14305
Delivering umbilical cord mesenchymal stem cell exosomes through hydrogel ameliorates vaginal atrophy in ovariectomized rats
Figure 3. The effects of ucMSC-Ex on VK2 cell proliferation, migration, and differentiation. (A) CCK-8 assay measuring VK2 cell viability at different concentrations; (B) Flow cytometry analysis of cell cycle after co-culture for 18 hours; (C) Quantification of S-phase cell proportion; (D) EdU assay detecting VK2 cell proliferation after 24 hours of co-culture; (E) Quantitative analysis of EdU-positive cells; (F) Scratch assay showing ucMSC-Ex promotion of VK2 cell migration; (G) Quantification of cell migration; (H) Normalized expression levels of differentiation-related genes in ucMSC-ex treated group compared to PBS group.