Research Paper Volume 15, Issue 23 pp 14172—14191

Figure 7. GP blocks tau hyperphosphorylation and Aβ overproduction in STP rats. (A) Western blot for tau phosphorylation levels at the sites of pS199, pT231, pS262, pS396, pS404, AT8 and total tau (tau-5) in rat hippocampus. (B–H) Quantification of the relative protein expression levels (pS199, pT231, pS262, pS396, pS404, AT8 and tau-5) after normalization to the β-actin signal. (I, J) ELISA assay of the levels of Aβ40 and Aβ42 in the rat hippocampus. (K) Western blots for APP, BACE1, ADAM10, PS1 and sAPPβ in rat hippocampus. (L–P) Quantification of the relative protein levels (BACE1, PS1, sAPPβ, ADAM10 and APP) after normalization to the β-actin signal. Data represent mean ± SEM, p-value significance is calculated from one-way ANOVA, n = 3. ^*p < 0.05, ^**p < 0.01 and ^***P < 0.001, Abbreviation: NS: no significance.