Research Paper Volume 15, Issue 23 pp 14172—14191

Figure 2. GP attenuated STP-induced tau phosphorylation and reduced Aβ generation in primary hippocampus neurons. (A) Western blot for tau phosphorylation levels at the sites of pS199, pT231, pS262, pS396, pS404, AT8 and total tau (Tau-5) in primary hippocampus neurons. (B–H) Quantification of the relative protein expression levels (pS199, pT231, pS262, pS396, pS404, AT8 and Tau-5) after normalization to the β-actin signal. (I, J) ELISA assay of the levels of Aβ40 and Aβ42 in rat primary hippocampal neurons. (K) Western blots for APP, BACE1, ADAM10, PS1 and sAPPβ in rat primary hippocampal neurons. (L–P) Quantification of the relative protein levels (BACE1, PS1, sAPPβ, ADAM10 and APP) after normalization to the β-actin signal. Data represent mean ± SEM, p-value significance is calculated from one-way ANOVA, n = 3. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 and ^****P < 0.0001. Abbreviation: NS: no significance.