Research Paper Volume 16, Issue 1 pp 1—14

Figure 2. Resveratrol treatment increased SIRT1 expression in adipose-derived progenitor cells (APCs) from young and older participants. (A) SIRT1 mRNA abundance measured after completion of the vehicle control during time course full time course (every 6h for 48 h) in young-APCs (green) vs. older-APCs (red). The effect of time, age and interactions was analyzed by linear mixed model. a: P<0.05 effect of time; b: P<0.05 effect of age; c: P<0.05 time by age interaction. (B) SIRT1 mRNA abundance measured after completion of the vehicle control (black) or RSV treatment (blue) at t = 0 in both young-APCs vs. older-APCs. APCs were synchronized with 30% fetal bovine serum (FBS) for two hrs and treated with vehicle for 12 hrs. RNA was extracted from APCs, harvested every six hrs from 12 to 60 hrs post-synchronization. Abundance of transcripts was quantified by RT-PCR and normalized to Peptidylprolyl isomerase A (PPIA). Data are presented as mean ± SEM.