Research Paper Volume 16, Issue 1 pp 1—14

Figure 1. mRNA expression of components of core clock mechanism are rhythmic in human adipose-derived progenitor cells (APCs) from young and older participants. APCs were synchronized with 30% fetal bovine serum (FBS) for two hrs and treated with vehicle for 12 hrs. RNA was extracted from APCs, harvested every six hrs from 12 to 60 hrs post-synchronization. Abundance of transcripts of core clock gene components was quantified by RT-PCR and normalized to Peptidylprolyl isomerase A (PPIA). Data are presented as mean ± SEM. When ARSER analysis detected a significant rhythm (P<0.05), symbols in the graphics represent oscillation in young (green), in older (red), or in both groups. BMAL1: brain and muscle ARNT-like protein-1, CLOCK: circadian locomotor output cycles kaput; PER1/2: period 1/2, CRY1/2: cytochrome 1/2, D site of albumin promoter (albumin D-box) binding protein, RORα: RAR related orphan receptor A; NR1D1/2, nuclear receptor subfamily 1 group D member 1/2.