Research Paper Volume 15, Issue 23 pp 14019—14038
Rewiring of IGF1 secretion and enhanced IGF1R signaling induced by co-chaperone carboxyl-terminus of Hsp70 interacting protein in adipose-derived stem cells provide augmented cardioprotection in aging-hypertensive rats
Figure 2. Colocalization of IGF1R with co-chaperone CHIP in rADSCs. (A) Level of IGF1Rβ in the membrane isolates was measured by Western blotting after overexpression of HA-vector/HA-CHIP wild-type for 24 h in rADSCs. (B) Cellular localization of CHIP and IGF1Rβ with or without CHIP overexpression in rADSCs. Scale bars = 20 μm. (C) Confocal microscope analysis to indicate the CHIP (green color-left) and IGF1Rβ (red color-middle) expression and colocalization (orange color-right) with or without CHIP overexpression in rADSCs. Scale bar = 10 μm. (D) Western blot analysis indicates that the cytoplasmic and nuclear extraction of various proteins expression, respectively. (N = 3; *p < 0.05; **p < 0.01 indicate significant differences).