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Research Paper Volume 15, Issue 22 pp 13542—13557

Long non-coding RNA EGOT is associated with 131iodine sensitivity and contributes to thyroid cancer progression by targeting miR-641/PTEN axis

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Figure 4. EGOT is able to sponge miR-641 in 131I-resistant TC cells. (A) The binding side prediction of EGOT and miR-641 in the ENCORI database. (B, C) The 131I -resistant TPC-1 and FTC-133 cells were treated with miR-641 mimic. (B) The qPCR analysis of miR-641 in the cells. (C) Luciferase reporter gene assays of EGOT luciferase activities. (D) The qPCR analysis of miR-641 in 131I -resistant TPC-1 and FTC-133 cells treated with EGOT siRNA. (E) The qPCR analysis of miR-641 in 131I -resistant TPC-1 and FTC-133 cells treated with EGOT overexpressing plasmid. (F) The direct interaction of EGOT and miR-641 was analyzed by RNA pull down. mean ± SD, ** P < 0.01. Experiments were repeated at least biological triplicates.