Research Paper Volume 16, Issue 1 pp 43—65

Figure 9. In vitro experiments for ASNS. (A) Relative expression of ASNS in PRAD and normal tissues in TCGA cohort. (B) ASNS was associated with worse BCRF survival of PRAD patients in TCGA cohort. (C) Human Protein Atlas revealed that ASNS protein expression was relatively higher in PRAD. (D, E) The expression levels of ASNS in PC-3 and DU145 cells were relatively higher than in RWPE-1 cells using qRT-PCR and western blot. (F) The CCK-8 assays demonstrated the correlations between ASNS and proliferation activity. (G) The EdU assays showed the correlations between ASNS and proliferation activity. (H) The transwell assays demonstrated the correlations between ASNS and migration capacity. (I) The invasion assays showed the correlations between ASNS and invasive competence. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001. Abbreviation: ns: no significance.